MDH1 anticorps (HRP)

N° du produit ABIN5596953

N° du produit (Fournisseur): 200-603-145-0100

L’anticorps Mouton Polyclonal anti-MDH1 a été validé pour WB, ELISA, IP et IHC. Il convient pour détecter MDH1 dans des échantillons de Porc.

Aperçu rapide pour MDH1 anticorps (HRP) (ABIN5596953)

Antigène: MDH1 (Malate Dehydrogenase 1, NAD (Soluble) (MDH1))

Reactivité: Porc

Hôte: Mouton

Clonalité: Polyclonal

Conjugué: Cet anticorp MDH1 est conjugé à/à la HRP

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunohistochemistry (IHC)

Détail du produit anti-MDH1 anticorps (HRP)

N° du produit (Fournisseur): 200-603-145-0100

Fournisseur: Rockland

Fonction: Malate Dehydrogenase Antibody Peroxidase Conjugated

Réactivité croisée (Details): Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum as well as purified and partially purified Malate Dehydrogenase [Pig Heart].

Attributs du produit: Synonyms: sheep anti-Malate Dehydrogenase HRP conjugated Antibody, peroxidase conjugated sheep anti-Malate Dehydrogenase Antibody, cytoplasmic, Cytosolic malate dehydrogenase

Purification: Malate Dehydrogenase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.

Immunogène:

Immunogen: Malate Dehydrogenase [Pig Heart]

Immunogen Type: Native Protein

Isotype: IgG

Information d'application

Indications d'application:

Immunohistochemistry Dilution: 1:50 - 1:250

Application Note: Anti-Malate Dehydrogenase Peroxidase Conjugated has been tested by western blot and is useful in ELISA and immunohistochemistry. Optimal titers should be obtained by researchers.

Western Blot Dilution: 1:1,000 - 1:5,000

Immunoprecipitation Dilution: 1:50

ELISA Dilution: 1:1,000 - 1:5,000

Other: User Optimized

Restrictions: For Research Use only

Stockage

Format: Lyophilized

Reconstitution:

Reconstitution Volume: 100 μL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Concentration: 1 mg/mL

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Preservative: 0.01 % (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Agent conservateur: Gentamicin sulfate

Précaution d'utilisation: This product contains Gentamicin sulfate: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C,-20 °C

Stockage commentaire: Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 

Date de péremption: 12 months

Détails sur MDH1

Antigène: MDH1 (Malate Dehydrogenase 1, NAD (Soluble) (MDH1))

Autre désignation: MDH1

Sujet: Background: Malate Dehydrogenase is an enzyme that reversibly catalyzes the oxidation of malate to oxaloacetate using the reduction of NAD+ to NADH. Malate Dehydrogenase is involved in gluconeogenesis, the synthesis of glucose from smaller molecules. There are two main isoforms in eukaryotic cells. One is found in the mitochondrial matrix the other is found in the cytoplasm.

ID gène: 396894

UniProt: P11708